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UPPGRADER™ Platform

UPPGRADER™

Our target ID platform to select promising novel E3 ligase

Query

INPUT
Specific novel E3L
Desired novel E3L profile
  • Cancer specific E3L
  • Tissue specific E3L
  • Lower Hematologic Tox. E3L
  • Universal E3L
Exp. level & pattern of CRBN, VHL in the certain tissues, cell subtypes, or diseases
Exp. level & pattern of CRBN, VHL in the certain in vitro cell lines
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UPPGRADER™(Target I.D. Solution for Novel E3L)

STEP ①
Analyze Exp. Level & Pattern of (Novel) E3L at Single Cell RNA

Analyze Open Source DB (GEO) or Self-Produced scRNA-seq Data

Bioinformatics
STEP ②
Verify Expression Level & Pattern of (Novel) E3L at Protein Level

Verify Open Source DB (Protein Atlas) & Proteomics related Publications or Self-Produced IHC Data

Biology
STEP ③
Validate Availability of the E3L as PROTAC E3L via Biology Study

Review Whether the E3L is Bona Fide Ubiqutin Ligase via Study of Prior Literatures or internal experiments

Biology
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Insight

OUTPUT
Promising novel E3L which fits to planned E3L profile & purpose
CRBN or VHL that would be more beneficial for planned PROTAC
Suitable cell lines for accurate in vitro test for planned PROTAC(Hedge negative false virto assay results by careful selection of cell line with proper expression of CRBV or VHL which PROTAC will employ)